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SOP-SC-006-E01-01.html

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  1. <!-- SOP-SC-006-E01-01 人脐带间充质干细胞原代二次贴壁操作记录(A4 竖版) -->
    2. 

  2. <div class="sop-record sop-a4 sop-sc-006" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
    3. 

  3.   <table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
    4. 

  4.     <colgroup>
    5. 

  5.       <col style="width:15%" />
    6. 

  6.       <col style="width:50%" />
    7. 

  7.       <col style="width:35%" />
    8. 

  8.     </colgroup>
    9. 

  9.     <tbody>
    10. 

  10.       <tr>
    11. 

  11.         <td rowspan="2" style="border:1px solid #334155; padding:6px; text-align:center; vertical-align:middle;">
    12. 

  12.           <img src="/sop-templates/SOP-SC-006-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
    13. 

  13.         </td>
    14. 

  14.         <td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, 'Songti SC', serif; font-size: 11pt; font-weight:700;">
    15. 

  15.           人脐带间充质干细胞原代二次贴壁操作记录
    16. 

  16.         </td>
    17. 

  17.         <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
    18. 

  18.           文件编码: SOP-SC-006-E01
    19. 

  19.         </td>
    20. 

  20.       </tr>
    21. 

  21.       <tr>
    22. 

  22.         <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
    23. 

  23.           版 本 号: 01
    24. 

  24.         </td>
    25. 

  25.       </tr>
    26. 

  26.     </tbody>
    27. 

  27.   </table>
    28. 

  28. 

  29.   <table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
    30. 

  30.     <colgroup>
    31. 

  31.       <col style="width:8.1%" />
    32. 

  32.       <col style="width:3.3%" />
    33. 

  33.       <col style="width:20.3%" />
    34. 

  34.       <col style="width:1.4%" />
    35. 

  35.       <col style="width:2.4%" />
    36. 

  36.       <col style="width:14.7%" />
    37. 

  37.       <col style="width:5.8%" />
    38. 

  38.       <col style="width:5.6%" />
    39. 

  39.       <col style="width:2.5%" />
    40. 

  40.       <col style="width:17.1%" />
    41. 

  41.       <col style="width:5.1%" />
    42. 

  42.       <col style="width:13.7%" />
    43. 

  43.     </colgroup>
    44. 

  44.     <tbody><tr class="sop-row-hidden sop-row-hidden">
    45. 

  45.         <td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 脐带MSC原代二次贴壁</td>
    46. 

  46.         <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
    47. 

  47.         <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;">&nbsp;</td>
    48. 

  48.       </tr><tr class="sop-row-hidden sop-row-hidden">
    49. 

  49.         <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
    50. 

  50.         <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
    51. 

  51.         <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
    52. 

  52.         <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
    53. 

  53.       </tr><tr class="sop-row-hidden sop-row-hidden">
    54. 

  54.         <td rowspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; vertical-align:middle; line-height:1.25;">试剂</td>
    55. 

  55.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">名称</td>
    56. 

  56.         <td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">生产厂家</td>
    57. 

  57.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">批号</td>
    58. 

  58.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">使用数量</td>
    59. 

  59.       </tr><tr class="sop-row-hidden sop-row-hidden">
    60. 

  60.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">磷酸盐缓冲液(PBS)</td>
    61. 

  61.         <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    62. 

  62.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    63. 

  63.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    64. 

  64.       </tr><tr class="sop-row-hidden sop-row-hidden">
    65. 

  65.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">MSC完全培养基</td>
    66. 

  66.         <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    67. 

  67.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    68. 

  68.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    69. 

  69.       </tr><tr class="sop-row-hidden sop-row-hidden">
    70. 

  70.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">重组胰酶</td>
    71. 

  71.         <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    72. 

  72.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    73. 

  73.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    74. 

  74.       </tr><tr class="sop-row-hidden sop-row-hidden">
    75. 

  75.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">消化液</td>
    76. 

  76.         <td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">-----</td>
    77. 

  77.         <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">制定配制记录</td>
    78. 

  78.         <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
    79. 

  79.       </tr><tr class="sop-row-hidden sop-row-hidden">
    80. 

  80.         <td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
    81. 

  81.         <td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
    82. 

  82.           <div>
    83. 

  83.             生物安全柜:
    84. 

  84.             <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW015</span>
    85. 

  85.             <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW022</span>
    86. 

  86.             <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
    87. 

  87.             <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
    88. 

  88.           </div>
    89. 

  89.           <div style="margin-top:6px;">
    90. 

  90.             离心机:
    91. 

  91.             <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
    92. 

  92.             <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
    93. 

  93.             <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
    94. 

  94.             <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
    95. 

  95.           </div>
    96. 

  96.         </td>
    97. 

  97.       </tr><tr class="sop-fill-row">
    98. 

  98.         <td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">干细胞原代<br />培养<br />(二次贴壁)</td>
    99. 

  99.         <td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
    100. 

  100.           <p style="margin:0 0 6px;">
    101. 

  101.             1. 观察细胞: <span class="sop-underline" style="text-align:left; min-width:160px;">&nbsp;</span>, 此时融合度达到90%以上, 综合考虑, 该批细胞于Day
    102. 

  102.             <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>进行二次贴壁.
    103. 

  103.           </p>
    104. 

  104.           <p style="margin:0 0 6px;">
    105. 

  105.             2. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用移液管轻轻吸出上清共
    106. 

  106.             <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 移入新的T175瓶中进行二次贴壁, 记为Day 0, 继续培养;
    107. 

  107.           </p>
    108. 

  108.           <p style="margin:0 0 6px;">
    109. 

  109.             3. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    110. 

  110.           </p>
    111. 

  111.           <p style="margin:0 0 6px;">
    112. 

  112.             4. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    113. 

  113.           </p>
    114. 

  114.           <p style="margin:0 0 6px;">
    115. 

  115.             5. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    116. 

  116.           </p>
    117. 

  117.           <p style="margin:0 0 6px;">
    118. 

  118.             6. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    119. 

  119.           </p>
    120. 

  120.           <p style="margin:0 0 6px;">
    121. 

  121.             7. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    122. 

  122.           </p>
    123. 

  123.           <p style="margin:0 0 6px;">
    124. 

  124.             8. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    125. 

  125.           </p>
    126. 

  126.           <p style="margin:0 0 6px;">
    127. 

  127.             9. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    128. 

  128.           </p>
    129. 

  129.           <p style="margin:0 0 6px;">
    130. 

  130.             10. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    131. 

  131.           </p>
    132. 

  132.           <p style="margin:0 0 6px;">
    133. 

  133.             11. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>执行SOP-SC-004-E01-01半换液, 继续培养;
    134. 

  134.           </p>
    135. 

  135.           <p style="margin:0;">
    136. 

  136.             12. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察到细胞局部融合度大于90%, 执行SOP-SC-005-E01-01重铺, 并做好重铺标记.
    137. 

  137.           </p>
    138. 

  138.         </td>
    139. 

  139.       </tr><tr class="sop-row-hidden sop-row-hidden">
    140. 

  140.         <td colspan="7" style="border:1px solid #334155; padding:8px;">每个培养瓶上皆清楚标示细胞批号</td>
    141. 

  141.         <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
    142. 

  142.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
    143. 

  143.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
    144. 

  144.         </td>
    145. 

  145.       </tr><tr class="sop-row-hidden sop-row-hidden">
    146. 

  146.         <td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
    147. 

  147.         <td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
    148. 

  148.         <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
    149. 

  149.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
    150. 

  150.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
    151. 

  151.         </td>
    152. 

  152.       </tr><tr class="sop-row-hidden sop-row-hidden">
    153. 

  153.         <td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
    154. 

  154.         <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
    155. 

  155.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
    156. 

  156.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
    157. 

  157.         </td>
    158. 

  158.       </tr><tr class="sop-row-hidden sop-row-hidden">
    159. 

  159.         <td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
    160. 

  160.         <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
    161. 

  161.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
    162. 

  162.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
    163. 

  163.         </td>
    164. 

  164.         <td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
    165. 

  165.         <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
    166. 

  166.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
    167. 

  167.           <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
    168. 

  168.         </td>
    169. 

  169.       </tr><tr class="sop-row-hidden sop-row-hidden">
    170. 

  170.         <td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
    171. 

  171.         <td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;">&nbsp;</td>
    172. 

  172.       </tr><tr class="sop-row-hidden sop-row-hidden">
    173. 

  173.         <td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
    174. 

  174.         <td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
    175. 

  175.         <td colspan="3" style="border:1px solid #334155; padding:10px;">生产主管/日期:</td>
    176. 

  176.       </tr></tbody>
    177. 

  177.   </table>
    178. 

  178. </div>
    179.