zkxw/docs/sop-templates/SOP-SC-003-E01-01.html

<!-- SOP-SC-003-E01-01 人脐带间充质干细胞原代培养操作记录(A4 竖版) -->
<div class="sop-record sop-a4 sop-sc-003" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
  <table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
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          <img src="/sop-templates/SOP-SC-003-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
        </td>
        <td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, 'Songti SC', serif; font-size: 12pt; font-weight:700;">
          人脐带间充质干细胞原代培养操作记录
        </td>
        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          文件编码: SOP-SC-003-E01
        </td>
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      <tr>
        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          版 本 号: 01
        </td>
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    </tbody>
  </table>

  <table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
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    <tbody><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 脐带MSC原代细胞培养</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="5" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">试剂</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">名称</td>
        <td colspan="5" style="border:1px solid #334155; padding:8px; text-align:center;">生产厂家</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">批号</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">使用数量</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px;">磷酸盐缓冲液(PBS)</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px;">MSC培养基</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px;">青/链霉素(双抗)</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px;">75%酒精</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
          <div>
            生物安全柜:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW015</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW022</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
          </div>
          <div style="margin-top:6px;">
            离心机:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
          </div>
        </td>
      </tr><tr class="sop-fill-row">
        <td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">脐带组织处理<br />及干细胞<br />原代培养</td>
        <td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
          <p style="margin:0 0 6px;">
            1. 组织来源: 接收的脐带物料编码<span class="sop-underline" style="text-align:left; min-width:140px;">&nbsp;</span>. 采集时间:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>24h内</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>超过24h</span>;
            入库编码: <span class="sop-underline" style="text-align:left; min-width:120px;">&nbsp;</span>;
          </p>
          <p style="margin:0 0 6px;">2. 将脐带组织转移至不锈钢碗中;</p>
          <p style="margin:0 0 6px;">
            3. 手术剪裁断脐带, 弃掉结扎部分, 截下一小段留样, 其余部分用清洗液漂洗
            <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>次;
          </p>
          <p style="margin:0 0 6px;">4. 取采集瓶中保存液10mL存于4℃冰箱留样, 留样标签应注明脐带条码, 样本类型, 操作人, 操作日期等;</p>
          <p style="margin:0 0 6px;">
            5. 将脐带在不锈钢碗内, 用清洗液漂洗
            <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>次, 清洗液无明显血色后, 转入培养皿;
          </p>
          <p style="margin:0 0 6px;">
            6. 用手术剪将脐带分成2~3cm的小段, 共计
            <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>段, 放入装有75%酒精的250mL离心瓶中浸泡30s后用清洗液冲洗干净.
          </p>
          <p style="margin:0 0 6px;">7. 用组织镊将脐动脉和脐静脉内的血丝清理掉并洗净;</p>
          <p style="margin:0 0 6px;">8. 由各段脐带组织剥取华通氏胶, 将剥好的华通氏胶放入一个盛有生理盐水的新培养皿内;</p>
          <p style="margin:0 0 6px;">9. 用手术剪将华通氏胶剪成2~4mm<sup>3</sup>大小的组织块, 将剪碎的组织块使用清洗液重悬并200G*5min离心, 取上清存于4℃冰箱待检, 样本标签应注明脐带条码, 样本类型, 操作人, 操作日期等信息;</p>
          <p style="margin:0 0 6px;">
            10. 离心后的组织沉淀共
            <span class="sop-underline" style="text-align:left; min-width:48px;">&nbsp;</span>mL, 接种
            <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>个T175, 每个T175培养瓶接种
            <span class="sop-underline" style="text-align:left; min-width:48px;">&nbsp;</span>mL, 接种完成后补加
            <span class="sop-underline" style="text-align:left; min-width:48px;">&nbsp;</span>mL原代细胞培养液.
          </p>
          <p style="margin:0;">11. 培养瓶上标记相关信息, 包括细胞来源、操作项目、操作时间、操作人等. 小心将培养瓶放入37℃, 5%CO<sub>2</sub>的培养箱中培养.</p>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="7" style="border:1px solid #334155; padding:8px;">每个培养瓶上皆清楚标示细胞批号</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
        <td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
        <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
        <td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
        <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
        <td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
        <td colspan="3" style="border:1px solid #334155; padding:10px;">主管/日期:</td>
      </tr></tbody>
  </table>
</div>