zkxw/docs/sop-templates/SOP-CC-002-E01-01.html

<!-- SOP-CC-002-E01-01 造血干细胞制备标准操作记录(A4 竖版) -->
<div class="sop-record sop-a4 sop-cc-002" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
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          <img src="/sop-templates/SOP-CC-002-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
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          造血干细胞制备标准操作记录
        </td>
        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          文件编码: SOP-CC-002-E01
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          版 本 号: 01
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  <table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
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    <tbody><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="5" style="border:1px solid #334155; padding:8px;">工序: 造血干细胞制备标准操作记录</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
        <td colspan="3" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
        <td colspan="3" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
        <td colspan="3" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
        <td colspan="9" style="border:1px solid #334155; padding:8px; vertical-align:top;">
          <div>
            电子天平:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW017</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:96px;">&nbsp;</span>
          </div>
          <div style="margin-top:6px;">
            高频热合仪:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW036</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:96px;">&nbsp;</span>
          </div>
          <div style="margin-top:6px;">
            低温离心机:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:96px;">&nbsp;</span>
          </div>
        </td>
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        <td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">造血干细胞<br />富集标准<br />操作记录</td>
        <td class="sop-fill-cell" colspan="9" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
          <p style="margin:0 0 6px;">
            1. 样本来源: 接收的脐血物料编码<span class="sop-underline" style="text-align:left; min-width:140px;">&nbsp;</span>.
            采集时间:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>24h内</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>超过24h</span>;
            入库编码: <span class="sop-underline" style="text-align:left; min-width:120px;">&nbsp;</span>;
          </p>
          <p style="margin:0 0 6px;">2. 脐血放入低温离心机的转子内, 并用隔板固定住脐血袋.</p>
          <p style="margin:0 0 6px;">
            3. 把装有脐血血袋的转子进行称重 <span class="sop-underline" style="text-align:left; min-width:64px;">&nbsp;</span> g, 加入HES <span class="sop-underline" style="text-align:left; min-width:64px;">&nbsp;</span> mL, 选择
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>去红去浆</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>只去浆</span>
            程序进行操作;
          </p>
          <p style="margin:0 0 6px;">4. 进行配平, 对称放入离心机.</p>
          <p style="margin:0 0 6px;">5. 脐血袋第一次离心420rmp, 5min,</p>
          <p style="margin:0 0 6px;">6. 离心结束后用分浆夹在白细胞层下1cm处夹紧, 进行分浆.</p>
          <p style="margin:0 0 6px;">7. 脐血袋第二次离心1500rmp, 5min.</p>
          <p style="margin:0 0 6px;">8. 离心结束后用分浆夹在白细胞层下1cm处夹紧, 让转移袋的一部分血浆回流后再进行分浆.</p>
          <p style="margin:0 0 6px;">9. 脐血袋第三次离心1500rmp, 5min.</p>
          <p style="margin:0 0 6px;">10. 离心结束后分浆夹在转移袋白细胞层上1cm处夹紧, 进行分浆. 在血浆全部流至导管后关闭血袋导管上的夹子.</p>
          <p style="margin:0;">11. 热合仪热合导管, 分开采血袋与转移袋.</p>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">采血袋后续操作</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">转移袋后续操作</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">后续操作记录编号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
        <td colspan="5" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
        <td colspan="4" style="border:1px solid #334155; padding:8px;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="5" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
        <td colspan="4" style="border:1px solid #334155; padding:8px;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
        <td colspan="2" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
        <td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒仪器</td>
        <td colspan="1" class="sop-option-cell sop-option-cell-wide" style="border:1px solid #334155; padding:8px 6px; white-space:nowrap;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
        <td colspan="9" style="border:1px solid #334155; padding:8px; min-height:56px; height:56px; vertical-align:top;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="3" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
        <td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
        <td colspan="2" style="border:1px solid #334155; padding:10px;">主管/日期:</td>
      </tr></tbody>
  </table>
</div>