zkxw/docs/sop-templates/SOP-SC-011-E01-01.html

<!-- SOP-SC-011-E01-01 外周血PBMC分离操作记录(A4 竖版) -->
<div class="sop-record sop-a4 sop-sc-011" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
  <table class="sop-header-table sop-row-hidden" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; margin-bottom:8px; flex-shrink:0;">
    <colgroup>
      <col style="width:15%" />
      <col style="width:48%" />
      <col style="width:37%" />
    </colgroup>
    <tbody>
      <tr>
        <td rowspan="2" style="border:1px solid #334155; padding:6px; text-align:center; vertical-align:middle;">
          <img src="/sop-templates/SOP-SC-011-logo.jpeg" alt="logo" width="48" height="48" style="display:block; margin:0 auto;" />
        </td>
        <td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; font-family: SimSun, 'Songti SC', serif; font-size: 12pt; font-weight:700;">
          外周PBMC分离操作记录
        </td>
        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          文件编码: SOP-SC-011-E01
        </td>
      </tr>
      <tr>
        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          版 本 号: 01
        </td>
      </tr>
    </tbody>
  </table>

  <table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
    <colgroup>
      <col style="width:8.1%" />
      <col style="width:3.3%" />
      <col style="width:20.2%" />
      <col style="width:1.4%" />
      <col style="width:2.4%" />
      <col style="width:14.7%" />
      <col style="width:5.7%" />
      <col style="width:5.7%" />
      <col style="width:2.4%" />
      <col style="width:17.1%" />
      <col style="width:5.1%" />
      <col style="width:13.9%" />
    </colgroup>
    <tbody><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 外周血PBMC分离</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="4" style="border:1px solid #334155; padding:3px 6px; text-align:center; vertical-align:middle; line-height:1.25;">试剂</td>
        <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">名称</td>
        <td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">生产厂家</td>
        <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">批号</td>
        <td colspan="2" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">使用数量</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">外周血</td>
        <td colspan="5" style="border:1px solid #334155; padding:3px 6px; text-align:center; line-height:1.25;">/</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">生理盐水</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">淋巴细胞分离液</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:3px 6px; line-height:1.25;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
          <div>
            生物安全柜:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW015</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW022</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
          </div>
          <div style="margin-top:6px;">
            离心机:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
          </div>
        </td>
      </tr><tr class="sop-fill-row">
        <td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">外周血<br />PBMC分离</td>
        <td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
          <p style="margin:0 0 6px;">
            1. 样本来源: 接收的外周血物料编码<span class="sop-underline" style="text-align:left; min-width:140px;">&nbsp;</span>. 采集时间:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>24h内</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>超过24h</span>;
            入库编码: <span class="sop-underline" style="text-align:left; min-width:120px;">&nbsp;</span>;
          </p>
          <p style="margin:0 0 6px;">2. 紫外灯照射30min;</p>
          <p style="margin:0 0 6px;">
            3. 血液总量<span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 分成
            <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>个50mL离心管进行处理;
          </p>
          <p style="margin:0 0 6px;">
            4. 使用离心机(
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            )650·g, 5 min, 升9降9, 结束后收集上清, 56℃水浴30min, 使用离心机(
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            )3000·g, 10 min, 取上清-20℃保存.
          </p>
          <p style="margin:0 0 6px;">5. 用生理盐水将下层溶液补至50mL;</p>
          <p style="margin:0 0 6px;">
            6. 铺血: 将混匀并稀释的外周血缓慢并小心地加到提前准备好的
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL,
            <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>管分离液中.
          </p>
          <p style="margin:0 0 6px;">
            7. 离心机(
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            )1200·g, 25 min, 升6降2;
          </p>
          <p style="margin:0 0 6px;">
            8. 取离心后的中间白膜层<span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 加生理盐水定容至50mL, 颠倒混匀, 离心机(
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            )930·g, 10 min, 升9降9;
          </p>
          <p style="margin:0 0 6px;">
            9. 弃去上清, 细胞合管, 生理盐水重悬细胞, 定容50mL, 混匀后计数
            <span class="sop-underline" style="text-align:left; min-width:64px;">&nbsp;</span>*10<sup>8</sup>个, 离心机(
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            )720·g, 8 min, 升9降9;
          </p>
          <p style="margin:0 0 6px;">
            10. 弃去上清, 生理盐水定容50mL, 离心机(
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW016</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW026</span>
            )720·g, 8 min, 升9降9;
          </p>
          <p style="margin:0 0 6px;">11. 离心结束后消毒离心管, 放入生物安全柜中, 根据需求进行冻存或细胞培养.</p>
          <p style="margin:0;">
            12. 对应细胞培养编号为: <span class="sop-underline" style="text-align:left; min-width:160px;">&nbsp;</span>;
            对应细胞冻存编号为: <span class="sop-underline" style="text-align:left; min-width:160px;">&nbsp;</span>.
          </p>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="7" style="border:1px solid #334155; padding:8px;">细胞后续培养用途</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="7" style="border:1px solid #334155; padding:8px;">后续培养编号</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
        <td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
        <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
        <td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
        <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
        <td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
        <td colspan="3" style="border:1px solid #334155; padding:10px;">主管/日期:</td>
      </tr></tbody>
  </table>
</div>