zkxw/docs/sop-templates/SOP-SC-004-E01-01.html

<!-- SOP-SC-004-E01-01 人脐带间充质干细胞原代培养半换液操作记录(A4 竖版) -->
<div class="sop-record sop-a4 sop-sc-004" style="font-family: 'PingFang SC','Microsoft YaHei',sans-serif; font-size: 10.5pt; color: #1e293b; line-height: 1.55; box-sizing: border-box; display: flex; flex-direction: column; height: 297mm; min-height: 297mm; padding: 16mm 15mm 20mm;">
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          人脐带间充质干细胞原代培养半换液操作记录
        </td>
        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          文件编码: SOP-SC-004-E01
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        <td style="border:1px solid #334155; padding:6px 10px; font-size: 12pt; vertical-align:middle;">
          版 本 号: 01
        </td>
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  </table>

  <table class="sop-body-table" style="width:100%; border-collapse:collapse; table-layout:fixed; border:1px solid #334155; flex:1 1 auto; height:100%; min-height:0;">
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    <tbody><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">工序: 脐带MSC原代细胞培养半换液</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">记录编号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px; min-height:28px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">细胞批号</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; white-space:nowrap;">日期</td>
        <td colspan="4" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="2" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">试剂</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">名称</td>
        <td colspan="5" style="border:1px solid #334155; padding:8px; text-align:center;">生产厂家</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">批号</td>
        <td colspan="2" style="border:1px solid #334155; padding:8px; text-align:center;">使用数量</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="2" style="border:1px solid #334155; padding:8px;">干细胞培养基</td>
        <td colspan="5" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
        <td colspan="2" class="sop-blank-cell" style="border:1px solid #334155; padding:8px;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">仪器<br />设备</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
          <div>
            生物安全柜:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW015</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW022</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
          </div>
          <div style="margin-top:6px;">
            离心机:
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW015</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>XW022</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>其他</span>
            <span class="sop-underline" style="text-align:left; min-width:80px;">&nbsp;</span>
          </div>
        </td>
      </tr><tr class="sop-fill-row">
        <td class="sop-fill-label" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle; height:100%;">干细胞原代<br />培养半换液</td>
        <td class="sop-fill-cell" colspan="11" style="border:1px solid #334155; padding:10px; vertical-align:top; height:100%;">
          <p style="margin:0 0 6px;">
            1. 原代培养48h内, 观察细胞
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>已经</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>还未</span>
            爬出, 进行补液;
          </p>
          <p style="margin:0 0 6px;">
            2. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻加入
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 将体系补至30mL;
          </p>
          <p style="margin:0 0 6px;">3. 静置细胞7天;</p>
          <p style="margin:0 0 6px;">
            4. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察细胞
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>已经</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>还未</span>
            爬出, 进行半换液;
          </p>
          <p style="margin:0 0 6px;">
            5. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 再加入等量体积的MSC完全培养基;
          </p>
          <p style="margin:0 0 6px;">
            6. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察细胞
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>已经</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>还未</span>
            爬出, 进行半换液;
          </p>
          <p style="margin:0 0 6px;">
            7. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 再加入等量体积的MSC完全培养基;
          </p>
          <p style="margin:0 0 6px;">
            8. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察细胞
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>已经</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>还未</span>
            爬出, 进行半换液;
          </p>
          <p style="margin:0 0 6px;">
            9. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 再加入等量体积的MSC完全培养基;
          </p>
          <p style="margin:0 0 6px;">
            10. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察细胞
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>已经</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>还未</span>
            爬出, 进行半换液;
          </p>
          <p style="margin:0 0 6px;">
            11. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 再加入等量体积的MSC完全培养基;
          </p>
          <p style="margin:0 0 6px;">
            12. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察细胞
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>已经</span>
            <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>还未</span>
            爬出, 进行半换液;
          </p>
          <p style="margin:0 0 6px;">
            13. 在培养瓶表面喷洒酒精后, 用无尘布擦拭放入生物安全柜中, 打开瓶盖, 使用10mL移液管轻轻吸弃上清
            <span class="sop-underline" style="text-align:left; min-width:56px;">&nbsp;</span>mL, 再加入等量体积的MSC完全培养基;
          </p>
          <p style="margin:0;">
            14. Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>观察到爬出的细胞局部融合度达到80%-90%, 准备进行重铺.
          </p>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">小结</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; vertical-align:top;">
          细胞于Day <span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>开始观察到爬出; Day<span class="sop-underline" style="text-align:left; min-width:40px;">&nbsp;</span>进行重铺,
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
          进行二次贴壁.
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="7" style="border:1px solid #334155; padding:8px;">每个培养瓶上是否皆清楚标示细胞批号</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td rowspan="3" style="border:1px solid #334155; padding:8px; text-align:center; vertical-align:middle;">清场</td>
        <td colspan="6" style="border:1px solid #334155; padding:8px;">未使用完试剂是否放入指定位置</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="6" style="border:1px solid #334155; padding:8px;">废液是否进行收集处理</td>
        <td colspan="5" class="sop-option-cell" style="border:1px solid #334155; padding:8px; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="4" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒操作间</td>
        <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
        <td colspan="5" style="border:1px solid #334155; padding:8px; white-space:nowrap;">是否清洁, 消毒生物安全柜与移液枪</td>
        <td colspan="1" class="sop-option-cell" style="border:1px solid #334155; padding:8px; white-space:nowrap; text-align:center;">
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>是</span>
          <span class="sop-option"><span class="sop-check" contenteditable="false" data-mce-contenteditable="false">&nbsp;</span>否</span>
        </td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td style="border:1px solid #334155; padding:8px; text-align:center;">备注</td>
        <td colspan="11" style="border:1px solid #334155; padding:8px; min-height:48px; height:48px; vertical-align:top;">&nbsp;</td>
      </tr><tr class="sop-row-hidden sop-row-hidden">
        <td colspan="4" style="border:1px solid #334155; padding:10px;">操作人/日期:</td>
        <td colspan="5" style="border:1px solid #334155; padding:10px;">复核人/日期:</td>
        <td colspan="3" style="border:1px solid #334155; padding:10px;">生产主管/日期:</td>
      </tr></tbody>
  </table>
</div>